Restriktionsverdau - restriction digest ( translation glossary) Genetik,Naturwissenschaften

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Deutsch Begriff oder Satz:	Restriktionsverdau
Englisch Übersetzung:	restriction digest
Eingetragen von:	nocciola

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1:46pm Dec 14, 2006

Übersetzungen Deutsch > Englisch [PRO]
Science - Genetik

Deutsch Begriff oder Satz: Restriktionsverdau

Listed under "Detection of PCR products" in the description of a molecular biology course. I am translating references/Zeugnisse.
Is this Restriction enzyme digestion?

Your help urgently required!
Thank you, Hazel

nocciola

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Deutschland

restriction digest

Erklärung:
See parallel German/English sources

http://www.biol.ethz.ch/IMB/groups/berger_group/Lectures/Pra... (see pp. 3, 4 - Table of Contents, Part B)

http://www.botanik.univie.ac.at/lehre/lehrveranstaltungen/ss...

and native English sources

http://www.biochem.ucl.ac.uk/bsm/nmr/protocols/protocols/dig...

http://intranet.bpc.ac.uk/courses/download/bmsi/co/catering/...
"Prepare a separate restriction digest for each presumptive positive PCR amplification, and amplification products from control Cyclospora cayetanensis and Eimeria tenella strains."

http://www.genetics.wustl.edu/fish_lab/frank/cgi-bin/fish/pr...
"Stages in protocol
(1) Restriction digest of PAC DNA
(2) ligation of restriction digests
(3) PCR amplification of ligation products
(4) Sequencing of PCR products
(5) Trim sequence of primer and vector sequence"

http://ysp.ucdavis.edu/Research06/HuJ/default.html
"Restriction digest: Specific restriction enzymes (Stu1, Pvu1, Dra1) were added along with their respective buffers to eluted PCR reactions and incubated at 37ºC for two to three hours. Afterwards, gel electrophoresis was conducted as with a regular PCR reaction."

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Note added at 2 hrs (2006-12-14 16:18:43 GMT)
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See also http://www.methodbook.net/dna/restrdig.html and http://preuss.bsd.uchicago.edu/protocols/enzyme.html - the longer version "restriction enzyme digest(ion)" is also used.

Ausgewählte Antwort von:

Steffen Walter
Deutschland

Hinweis von Fragesteller an den Antwortenden

Thank you very much for your wonderful support!
4 KudoZ-Punkte wurden für diese Antwort vergeben

ZUSAMMENFASSUNG ALLER ÜBERSETZUNGEN (ENGLISCH)

4 +4

restriction digest

Steffen Walter

Antworten

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restriction digest

Erklärung:
See parallel German/English sources

http://www.biol.ethz.ch/IMB/groups/berger_group/Lectures/Pra... (see pp. 3, 4 - Table of Contents, Part B)

http://www.botanik.univie.ac.at/lehre/lehrveranstaltungen/ss...

and native English sources

http://www.biochem.ucl.ac.uk/bsm/nmr/protocols/protocols/dig...

http://intranet.bpc.ac.uk/courses/download/bmsi/co/catering/...
"Prepare a separate restriction digest for each presumptive positive PCR amplification, and amplification products from control Cyclospora cayetanensis and Eimeria tenella strains."

http://www.genetics.wustl.edu/fish_lab/frank/cgi-bin/fish/pr...
"Stages in protocol
(1) Restriction digest of PAC DNA
(2) ligation of restriction digests
(3) PCR amplification of ligation products
(4) Sequencing of PCR products
(5) Trim sequence of primer and vector sequence"

http://ysp.ucdavis.edu/Research06/HuJ/default.html
"Restriction digest: Specific restriction enzymes (Stu1, Pvu1, Dra1) were added along with their respective buffers to eluted PCR reactions and incubated at 37ºC for two to three hours. Afterwards, gel electrophoresis was conducted as with a regular PCR reaction."

--------------------------------------------------
Note added at 2 hrs (2006-12-14 16:18:43 GMT)
--------------------------------------------------

See also http://www.methodbook.net/dna/restrdig.html and http://preuss.bsd.uchicago.edu/protocols/enzyme.html - the longer version "restriction enzyme digest(ion)" is also used.

Steffen Walter
Deutschland
Muttersprache: Deutsch
PRO-Punkte in Kategorie: 4

Hinweis von Fragesteller an den Antwortenden

Thank you very much for your wonderful support!

Hinweise an den Beantworter der Frage

Fragesteller: Thank you very much for your help and all the background information. Like Mr. Sampat , I was astounded with all the research you did!! Today I joined proz. However, I am afraid that I won't be able to help people like you have helped me because I am fairly new to professional translation! Hazel (Nocciola)

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Deutsch: Restriktionsverdau

Englisch translation: restriction digest